Publications

2023
25. A. H. Kachroo (2023). Decoding human genetic variation using a synthetic paradigm. Nature Reviews Genetics.
doi: https://doi.org/10.1038/s41576-023-00682-x pmid: 38057547
24. S. Sultana, M. Abdullah, Jianhui Li, M. Hochstrasser, A. H. Kachroo (2023). Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeast. Genetics.
doi: 10.1093/genetics/iyad117 pmid: 37364278 bioRxiv: https://doi.org/10.1101/2022.06.20.496808
23. M. Abdullah, B. M. Greco, J. M. Laurent, R. K. Garge, D. R. Boutz, M. Vandeloo, E. M. Marcotte, A. H. Kachroo (2023). Rapid, scalable, combinatorial genome engineering by Marker-less Enrichment and Recombination of Genetically Engineered loci in yeast. Cell Reports Methods.
doi: 10.1016/j.crmeth.2023.100464 pmid: 37323580 bioRxiv: https://doi.org/10.1101/2022.06.17.496490

2022
22. A. H. Kachroo, M. Vandeloo, B. M. Greco, M. Abdullah (2022). Humanized yeast to model human biology, disease and evolution. Disease Models & Mechanisms.
doi: 10.1242/dmm.049309 pmid: 35661208

2021
21. R. K. Garge, H. J. Cha, C. Lee, J. D. Gollihar, A. H. Kachroo, J. B. Wallingford, E. M. Marcotte (2021). Discovery of new vascular disrupting agents based on evolutionarily conserved drug action, pesticide resistance mutations, and humanized yeast. Genetics.
doi: 10.1093/genetics/iyab101 pmcid: PMC8633126 bioRxiv: 10.1101/2020.09.15.298828

2020
20. R. K. Garge, J. M. Laurent, A. H. Kachroo, E. M. Marcotte (2020). Systematic humanization of the yeast cytoskeleton discerns functionally replaceable from divergent human genes. Genetics.
doi: 10.1534/genetics.120.303378 pmcid: PMC7404242 bioRxiv: 10.1101/2019.12.16.878751
19. J. M. Laurent, R. K. Garge, A. I. Teufel, C. O. Wilke, A. H. Kachroo, E. M. Marcotte* (2020). Humanization of yeast genes with multiple human orthologs reveals functional divergence between paralogs. PLoS Biology.
doi: 10.1371/journal.pbio.3000627 pmcid: PMC7259792 bioRxiv: 10.1101/668335

2019
18. A. I. Teufel, M. M. Johnson, J. M. Laurent, A. H. Kachroo, E. M. Marcotte, C. O. Wilke (2019). The many nuanced evolutionary consequences of duplicated genes. Molecular Biology and Evolution.
doi: 10.1093/molbev/msy210 pmcid: PMC6409435 bioRxiv: 10.1101/366971

2018
17. A. Akhmetov, J. M. Laurent, J. Gollihar, E. C. Gardner, R. K. Garge, A. D. Ellington, A. H. Kachroo, E. M. Marcotte (2018). Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9. Bio-protocol.
doi: 10.21769/BioProtoc.2765 pmcid: PMC5951413

2017
16. A. H. Kachroo, J. M. Laurent$, A. Akhmetov, M. Szilagyi-Jones, C. D. McWhite, A. Zhao, E. M. Marcotte (2017). Systematic bacterialization of yeast genes identifies a near-universally swappable pathway. eLife.
doi: 10.7554/eLife.25093 pmcid: PMC5536947

2016
15. J. M. Laurent, J. H. Young, A. H. Kachroo, E. M. Marcotte (2016). Efforts to make and apply humanized yeast. Brief Funct. Genomics 348:921–925.
doi: 10.1093/bfgp/elv041 pmcid: PMC4803062

2015
14. A. H. Kachroo, J. M. Laurent, C. M. Yellman, A. G. Meyer, C. O. Wilke, E. M. Marcotte (2015). Systematic humanization of yeast genes reveals conserved functions and genetic modularity. Science 348:921–925.
doi: 10.1126/science.aaa0769 pmcid: PMC4718922
13. M. Jayaram, C. H. Ma, A. H. Kachroo, P. A. Rowley, P. Guga, H. F. Fan, Y. Voziyanov (2015). An Overview of Tyrosine Site‐specific Recombination - From an Flp Perspective. Mobile DNA III.
doi: 10.1128/microbiolspec.MDNA3-0021-2014 pmid: 26350308
12. Yen Ting Liu, S. Sau, C. H. Ma, A. H. Kachroo, P. A. Rowley, K. M. Chang, H. F. Fan, M. Jayaram (2015). The Partitioning and Copy Number Control Systems of the Selfish Yeast Plasmid - An Optimized Molecular Design for Stable Persistence in Host Cells. Microbiology Spectrum.
doi: 10.1128/microbiolspec.PLAS-0003-2013 pmcid: PMC4274994
11. B. Cannon, A. H. Kachroo, I. Jarmoskaite, M. Jayaram, R. Russell (2015). Hexapeptides that inhibit processing of branched DNA structures induce a dynamic ensemble of holliday junction conformations. Journal of Biological Chemistry.
doi: 10.1074/jbc.M115.663930 pmcid: PMC4566245
10. P. A. Rowley, A. H. Kachroo, C. H. Ma, A. D. Maciaszek, P. Guga, M. Jayaram (2015). Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination. Nucleic Acids Research.
doi: 10.1093/nar/gkv513 pmcid: PMC4499138

2014
9. Yufeng Qian, A. H. Kachroo, C. M. Yellman, E. M. Marcotte, K. A. Johnson (2014). Yeast cells expressing the human mitochondrial DNA polymerase reveal correlations between polymerase fidelity and human disease progression. Journal of Biological Chemistry.
doi: 10.1074/jbc.M113.526418 pmcid: PMC3937665

2013
8. P. A. Rowley, A. H. Kachroo, M. Jayaram (2013). Selfish DNA. Brenner's Encyclopedia of Genetics.
doi: 10.1016/B978-0-12-374984-0.01394-2

2012
7. JN. Kalyani, N. Ramachandra, A. H. Kachroo, S. Mahadevan, H. S. Savithri (2012). Functional analysis of the genes encoding diaminopropionate ammonia lyase in Escherichia coli and Salmonella enterica serovar Typhimurium. Journal of Bacteriology.
doi: 10.1128/JB.01362-12 pmcid: PMC3458667

2010
6. P. A. Rowley, A. H. Kachroo, C. H. Ma, A. D. Maciaszek, P. Guga, M. Jayaram (2010). Electrostatic suppression allows tyrosine site-specific recombination in the absence of a conserved catalytic arginine. Journal of Biological Chemistry.
doi: 10.1074/jbc.M110.112292 pmcid: PMC2906291
5. A. H. Kachroo, P. A. Rowley, C. H. Ma, A. D. Maciaszek, P. Guga, M. Jayaram (2010). Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate. Nucleic Acids Research.
doi: 10.1093/nar/gkq466 pmcid: PMC2965224

2009
4. A. H Kachroo, M. Jayaram, P. A. Rowley (2009). Metabolic engineering without plasmids. Nature Biotechnology.
doi: 10.1038/nbt0809-729 pmid: 19668179
3. C. H. Ma, A. H Kachroo, A. Macieszak, T. Y. Chen, P. Guga, M. Jayaram (2009). Reactions of Cre with methylphosphonate DNA - Similarities and contrasts with Flp and vaccinia topoisomerase. PLoS One.
doi: 10.1371/journal.pone.0007248 pmcid: PMC2747268

2007
2. A. H. Kachroo, A. K. Kancherla, NS. Singh, U. Varshney, S. Mahadevan (2007). Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose. Molecular Microbiology.
doi: 10.1111/j.1365-2958.2007.05999.x pmid: 18028317

2004
1. A. H. Kachroo (2004). Order in stress-Lessons from the inanimate world. Journal of Biosciences.
doi: 10.1007/BF02712104 pmid: 15625389