Publications

2023
24. S. Sultana$. M. Abdullah$, Jianhui Li, M. Hochstrasser, A. H. Kachroo* (2023). Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeast. Genetics.
doi: 10.1093/genetics/iyad117 pmid: 37364278 bioRxiv: https://doi.org/10.1101/2022.06.20.496808
23. M. Abdullah, B. M. Greco, J. M. Laurent, R. K. Garge, D. R. Boutz, M. Vandeloo, E. M. Marcotte, A. H. Kachroo* (2023). Rapid, scalable, combinatorial genome engineering by Marker-less Enrichment and Recombination of Genetically Engineered loci in yeast. Cell Reports Methods.
doi: 10.1016/j.crmeth.2023.100464 pmid: 37323580 bioRxiv: https://doi.org/10.1101/2022.06.17.496490

2022
22. A. H. Kachroo*, M. Vandeloo, B. M. Greco, M. Abdullah (2022). Humanized yeast to model human biology, disease and evolution. Disease Models & Mechanisms.
doi: 10.1242/dmm.049309 pmid: 35661208

2021
21. R. K. Garge, H. J. Cha, C. Lee, J. D. Gollihar, A. H. Kachroo, J. B. Wallingford*, E. M. Marcotte* (2021). Discovery of new vascular disrupting agents based on evolutionarily conserved drug action, pesticide resistance mutations, and humanized yeast. Genetics.
doi: 10.1093/genetics/iyab101 pmcid: PMC8633126 bioRxiv: 10.1101/2020.09.15.298828

2020
20. R. K. Garge, J. M. Laurent, A. H. Kachroo*, E. M. Marcotte* (2020). Systematic humanization of the yeast cytoskeleton discerns functionally replaceable from divergent human genes. Genetics.
doi: 10.1534/genetics.120.303378 pmcid: PMC7404242 bioRxiv: 10.1101/2019.12.16.878751
19. J. M. Laurent, R. K. Garge, A. I. Teufel, C. O. Wilke, A. H. Kachroo*, E. M. Marcotte* (2020). Humanization of yeast genes with multiple human orthologs reveals functional divergence between paralogs. PLoS Biology.
doi: 10.1371/journal.pbio.3000627 pmcid: PMC7259792 bioRxiv: 10.1101/668335

2019
18. A. I. Teufel, M. M. Johnson, J. M. Laurent, A. H. Kachroo, E. M. Marcotte, C. O. Wilke* (2019). The many nuanced evolutionary consequences of duplicated genes. Molecular Biology and Evolution.
doi: 10.1093/molbev/msy210 pmcid: PMC6409435 bioRxiv: 10.1101/366971

2018
17. A. Akhmetov, J. M. Laurent, J. Gollihar, E. C. Gardner, R. K. Garge, A. D. Ellington, A. H. Kachroo*, E. M. Marcotte* (2018). Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9. Bio-protocol.
doi: 10.21769/BioProtoc.2765 pmcid: PMC5951413

2017
16. A. H. Kachroo$*, J. M. Laurent$, A. Akhmetov, M. Szilagyi-Jones, C. D. McWhite, A. Zhao, E. M. Marcotte* (2017). Systematic bacterialization of yeast genes identifies a near-universally swappable pathway. eLife.
doi: 10.7554/eLife.25093 pmcid: PMC5536947

2016
15. J. M. Laurent, J. H. Young, A. H. Kachroo, E. M. Marcotte* (2016). Efforts to make and apply humanized yeast. Brief Funct. Genomics 348:921–925.
doi: 10.1093/bfgp/elv041 pmcid: PMC4803062

2015
14. A. H. Kachroo, J. M. Laurent, C. M. Yellman, A. G. Meyer, C. O. Wilke, E. M. Marcotte* (2015). Systematic humanization of yeast genes reveals conserved functions and genetic modularity. Science 348:921–925.
doi: 10.1126/science.aaa0769 pmcid: PMC4718922
13. M. Jayaram*, C. H. Ma, A. H. Kachroo, P. A. Rowley, P. Guga, H. F. Fan, Y. Voziyanov (2015). An Overview of Tyrosine Site‐specific Recombination - From an Flp Perspective. Mobile DNA III.
doi: 10.1128/microbiolspec.MDNA3-0021-2014 pmid: 26350308
12. Yen Ting Liu, S. Sau, C. H. Ma, A. H. Kachroo, P. A. Rowley, K. M. Chang, H. F. Fan, M. Jayaram* (2015). The Partitioning and Copy Number Control Systems of the Selfish Yeast Plasmid - An Optimized Molecular Design for Stable Persistence in Host Cells. Microbiology Spectrum.
doi: 10.1128/microbiolspec.PLAS-0003-2013 pmcid: PMC4274994
11. B. Cannon$, A. H. Kachroo$, I. Jarmoskaite, M. Jayaram*, R. Russell* (2015). Hexapeptides that inhibit processing of branched DNA structures induce a dynamic ensemble of holliday junction conformations. Journal of Biological Chemistry.
doi: 10.1074/jbc.M115.663930 pmcid: PMC4566245
10. P. A. Rowley$, A. H. Kachroo$, C. H. Ma, A. D. Maciaszek, P. Guga, M. Jayaram* (2015). Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination. Nucleic Acids Research.
doi: 10.1093/nar/gkv513 pmcid: PMC4499138

2014
9. Yufeng Qian, A. H. Kachroo, C. M. Yellman, E. M. Marcotte, K. A. Johnson* (2014). Yeast cells expressing the human mitochondrial DNA polymerase reveal correlations between polymerase fidelity and human disease progression. Journal of Biological Chemistry.
doi: 10.1074/jbc.M113.526418 pmcid: PMC3937665

2013
8. P. A. Rowley$, A. H. Kachroo$, M. Jayaram* (2013). Selfish DNA. Brenner's Encyclopedia of Genetics.
doi: 10.1016/B978-0-12-374984-0.01394-2

2012
7. JN. Kalyani, N. Ramachandra, A. H. Kachroo, S. Mahadevan, H. S. Savithri* (2012). Functional analysis of the genes encoding diaminopropionate ammonia lyase in Escherichia coli and Salmonella enterica serovar Typhimurium. Journal of Bacteriology.
doi: 10.1128/JB.01362-12 pmcid: PMC3458667

2010
6. P. A. Rowley, A. H. Kachroo, C. H. Ma, A. D. Maciaszek, P. Guga, M. Jayaram* (2010). Electrostatic suppression allows tyrosine site-specific recombination in the absence of a conserved catalytic arginine. Journal of Biological Chemistry.
doi: 10.1074/jbc.M110.112292 pmcid: PMC2906291
5. A. H. Kachroo, P. A. Rowley, C. H. Ma, A. D. Maciaszek, P. Guga, M. Jayaram* (2010). Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate. Nucleic Acids Research.
doi: 10.1093/nar/gkq466 pmcid: PMC2965224

2009
4. A. H Kachroo, M. Jayaram, P. A. Rowley* (2009). Metabolic engineering without plasmids. Nature Biotechnology.
doi: 10.1038/nbt0809-729 pmid: 19668179
3. C. H. Ma, A. H Kachroo, A. Macieszak, T. Y. Chen, P. Guga, M. Jayaram* (2009). Reactions of Cre with methylphosphonate DNA - Similarities and contrasts with Flp and vaccinia topoisomerase. PLoS One.
doi: 10.1371/journal.pone.0007248 pmcid: PMC2747268

2007
2. A. H. Kachroo, A. K. Kancherla, NS. Singh, U. Varshney, S. Mahadevan* (2007). Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose. Molecular Microbiology.
doi: 10.1111/j.1365-2958.2007.05999.x pmid: 18028317

2004
1. A. H. Kachroo* (2004). Order in stress-Lessons from the inanimate world. Journal of Biosciences.
doi: 10.1007/BF02712104 pmid: 15625389